NHS-Activated Silver Nanoparticles Conjugation Kit
Description :
Nanodiagnostics NHS-activated Silver Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.
The kits contain ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied in the kit to generate your conjugate.
The precisely engineered silver surface coat on our NHS ester-activated silver nanoparticles results in high conjugation efficiency and stable conjugates while minimizing non-specific binding in your assay.
Kit Components
Features & Benefits
Description :
Nanodiagnostics NHS-activated Silver Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugation of proteins and other primary amine-containing ligands to silver nanoparticles.
The kits contain ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied in the kit to generate your conjugate.
The precisely engineered silver surface coat on our NHS ester-activated silver nanoparticles results in high conjugation efficiency and stable conjugates while minimizing non-specific binding in your assay.
Kit Components
- • 10nm NHS-Activated Silver Nanoparticles (lyophilized)
- • Protein Resuspension Buffer
- • Reaction Buffer
- • Quencher Solution
Features & Benefits
- • Results in covalently bound ligand and more stable conjugate
- • Fast and convenient one-step conjugation reaction with no pre-activation requirements
- • Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
- • Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
80nm NHS-Activated Silver Nanoparticle Conjugation Kit (3 reactions)
This kit contains enough reagents for conjugation of 3 x 10µg of protein.
Specifications
Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between silver surface and NHS-group) Core diameter: 80nm Concentration: OD=50 (when dissolved to a final volume of 100µl) Particles per ml: ~1.4E+11 (when dissolved to a final volume of 100µl) Absorbance (λmax): 440-480nm
CATALOG NUMBER PACK SIZE
| RN-Ag-NHSkit-80-3x10µg | 3x10µg |
| RN-Ag-NHSkit-80-10x10µg | 10x10µg |
| RN-Ag-NHSkit-80-100µg | 100µg |
80nm NHS-Activated Silver Nanoparticle Conjugation Kit (10 reactions)
This kit contains enough reagents for conjugation of 10 x 10µg of protein.
Specifications
Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between silver surface and NHS-group) Core diameter: 80nm Concentration: OD=50 (when dissolved to a final volume of 100µl) Particles per ml: ~1.4E+11 (when dissolved to a final volume of 100µl) Absorbance (λmax): 440-480nm
80nm NHS-Activated Silver Nanoparticle Conjugation Kit (MIDI Kit)
This kit contains enough reagents for conjugation of 100µg of protein.
Specifications
Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between silver surface and NHS-group) Core diameter: 80nm Concentration: OD=50 (when dissolved to a final volume of 100µl) Particles per ml: ~1.4E+11 (when dissolved to a final volume of 100µl) Absorbance (λmax): 440-480nm